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Extra info for Advances in lipid research. Vol.22, 1987
Tiffany (unpublished). ^Carbon number of acid/carbon number of alcohol. Lipid Secretion of the Meibomian Glands 35 principal esters of cholesterol and cholestanol are present in the same proportions as the a-Cis, a-Ci7, and a-Ci9 esters of dihydrolanosterol identified by GLC (Tiffany and Marsden, 1982). The MPs of lipid standards, of the synthetic esters and of the individual WE isolated from rabbit meibomian secretion are all sharp. However, the results in Table III include upper and lower limits.
Some further preference for anteiso-branched primer rather than isobranched also seems to exist in rabbit, since few iso acids or alcohols are found in the native secretion even though several such precursors are presumably available in vivo. It had been noted on analysis of the fatty acids and alcohols of rabbit secretion (Tiffany, 1979) that the dominant alcohol was a-C27 : 0 whereas the dominant long-chain acid was a-C25 : 0. This led to the suggestion that reduction of acids in vivo might involve chain elongation.
Differences in mean chain length and degree of unsaturation between sebum and meibomian samples may account for some of the variation in viscosities found. Despite its obvious importance in spreading and maintaining the tear film, no studies on surface (two-dimensional) viscosity of meibomian lipid films seem to have been reported. C. HYDRATION AND SOLUBILITY IN WATER Brown and Dervichian (1969a) melted meibomian oil repeatedly in the presence of water, but found no change in appearance or MP, indicating no tendency to absorb water and swell to form one or more hydrated phases.