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By Basil J Nikolau; Eve Syrkin Wurtele

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Extra info for Concepts in plant metabolomics : [derived from presentations made at the 3rd International Congress of Plant Metabolomics, which was held in 2004 at Iowa State University, Ames, Iowa]

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2003). , 2001a). For instance, the equations in Figure 3-1C sequentially ask about the loci controlling the accumulation of the whole pathway (A–H), the accumulation of only those compounds on the right side (F–H), and the accumulation of those on the left side (D, E). These 3. Metabolomics and QTL Analysis 35 A A) 1 B 2 D C 5 3 4 E B) 6a H 6b G A, B, C … H C) ∑ i i= A D) F D E H E i=F i=D ∑i ∑i G H E E) ∑i i=D H ∑i i =C Figure 3-1. Metabolomics Variables for QTL Mapping. A. A hypothetical biosynthetic pathway is shown.

1 Plant material Nicotiana tabacum L. var. , 2003). Plant organs of 12 simultaneously grown plants were harvested 3 months after germination. All mature, non-senescent leafs were immediately shock-frozen in liquid nitrogen. The root systems were washed sand-free under tap water, shortly dried on filter paper, and subsequently frozen in liquid nitrogen. The complete root and leaf batches of 12 plants were homogenized separately in precooled mortars while permanently being kept under liquid nitrogen.

Other ways in which epistatic interactions may occur in biosynthetic pathways is if two proteins physically interact such that the variants from each parent prefer interacting with each other such as might occur in metabolic channeling. This will lead to any recombinant progeny between the loci having lower efficiencies and less compound accumulation. Taken 3. Metabolomics and QTL Analysis 41 together, this suggests that epistatic interactions should be expected in metabolomics QTL mapping projects.

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