By Thomas Kramps, Knut Elbers
This quantity presents an summary of the sector and functional tricks for vaccinologists in academia and undefined. Chapters supply protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and supply, and preclinical and medical improvement. Written within the hugely profitable Methods in Molecular Biology series structure, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, simply reproducible laboratory protocols, and tips about troubleshooting and heading off recognized pitfalls.
Authoritative and state of the art, RNA Vaccines: tools and Protocols goals to elevated collaboration on RNA vaccines among simple and utilized scientists in academia, govt, and to boost destiny strategies for today’s challenges.
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Additional resources for RNA Vaccines: Methods and Protocols
Meyers G, Tautz N, Becher P, Thiel HJ, Kümmerer BM (1996) Recovery of cytopathogenic and noncytopathogenic bovine viral diarrhea viruses from cDNA constructs. Meyers G, Thiel HJ, Rümenapf T (1996) Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles. J Virol 70(6): 3478–3487 19. Rice CM, Grakoui A, Galler R, Chambers TJ (1989) Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation.
Accordingly, these constructs allow the recovery of infectious virus particles. As presented above, introduction of the in vitro- transcribed recombinant RNA into a cell via transfection starts its autonomous replication leading to release of infectious viruses that after amplification in tissue culture serve as vaccine. Upon Self-Replicating RNA 27 administration, the immune response is triggered because the vaccine virus mimics all steps of a field virus infection but without induction of significant symptoms.
As a matter of fact, basically all approaches using self-replicating RNA for vaccination employ packaging of the RNA into virions or virus-like particles. The reason for this preference over naked or stabilized RNA is based on the extraordinary performance of the viral infection machinery resulting in highly efficient delivery of the RNA into cells. Since self-replicating RNAs derived from viral genomes exhibit the intrinsic property for specific packaging into viral particles, the use of this strategy is easy and straight forward.